Detection of rol B and rol C Genes in Callus Tissues Derived from Co-cultivation of Carrot (Daucus carota L.) Cell Suspension With pRi

Cell suspension cultures were produced from stem calli of carrot seedlings. Exposure of suspension of 3.4 x 105 cell ml-1 to 250 V/ 5 msec. stimulate their divisions producing 168 callus primordia, which developed to culture of callus. Whereas exposure of the mixture of cell suspension with 50 l of pRi plasmid, which isolated from Agrobacterium rhizogenes R1601 promoted the sequences of cellular division producing 232 callus primordia successed in forming cultures of callus as well. Addition of PEG (Mwt.6000) solution to cell suspension-plasmid mixture accelerate the colony formation which developed to callus culture as well. Data of the amplified (PCR) DNA electrophoresis demonstrated the development of two bands represented the rol B and rol C genes. Their molecular weight was 850 bp and 650 bp which are similar to the m wt. of the used primers. These results demonstrated the inclusion of these genes in genomic DNA of produced callus