Effect of β-Tri-calcium Phosphate in Both Its Putty and Granular Forms in Treating Bone Defects in Sheep
Abstract
The present study aims to evaluate and compare the effect of -tri-calcium phosphate granules and Putty on the healing of bony defects after induced injury on the tibia bone of sheep. Materials and Methods: Four healthy local breed male sheep all the sheep from the same farm. Four intervals were performed (2 weeks, 4 weeks, 6 weeks, 8 weeks). One tibia in each sheep was operated on at two two-week interval periods between each surgery (one surgery on each sheep making a total observation period of eight weeks). After two weeks of the last operation, the animals were euthanized based on a scheduled timeline. Results: The study reveals significant changes in osteoblasts, osteocytes, new capillaries, and histomorphometric measurements of bone marrow space over time, The study presents histological sections of sheep treated with granules and putty, revealing changes in bone marrow space histomorphometry and the presence of bone cells and capillaries. Conclusion: Although both kinds of -TCP putty and granules considerably improved bone fracture healing, the -TCP putty demonstrated a better and faster healing ability than the granules, with a marked improvement in the bone marrow area.
References
- Ahlhelm M, Latorre SH, Mayr HO, Storch C, Freytag C, Werner D, Schwarzer-Fischer E, Seidenstcker M. Mechanically stable -TCP structural hybrid scaffolds for potential bone replacement. J Compos Sci. 2021;5(10):281.
- Albalooshy A, Duggal M, Vinall-Collier K, Drummond B, Day P. The outcomes of auto-transplanted premolars in the anterior maxilla following traumatic dental injuries. Dental Traumatology. 2023;39(1):1-10.
- Balaji VR, Manikandan D, Ramsundar A. Bone grafts in periodontics. Matrix Sci Med. 2020;4(3):57-63.
- Bohner M, Santoni BLG, Dbelin N. -tricalcium phosphate for bone substitution: Synthesis and properties. Acta Biomater. 2020;113:2341.
- Dorozhkin SV. Self-setting calcium orthophosphate formulations: cements, concretes, puttys and putties. Int J Mater Chem. 2011;1(1):1-48.
- Garcia DC, Mingrone LE, S MJC. Evaluation of osseointegration and bone healing using pure-phase -tcp ceramic implant in bone critical defects. A systematic review. Front Vet Sci. 2022;9:859920.
- Hussein AA, A Taqa G. The impact of natural calcium carbonate and Ubiquinone on bone mineral density in rabbits. J Appl Vet Sci. 2021;6(4):15-22.
- Jose N, Sriramineni A, Mahendra J, Abirami N. Alloplastic putty bone graft-A short review. J Indian Dent Assoc. 2021. https://doi.org/10.37841/jidam_2021_V8_I1_08
- Kazemi, M., Dehghan, M.M. and Azami, M., (2019). Biological evaluation of porous nanocomposite scaffolds based on strontium substituted -TCP and bioactive glass: An in vitro and in vivo study. Materials Science and Engineering: C, 105, p.110071.
- Lopera-Echavarra AM, Medrano-David D, Lema-Perez AM, Araque-Marn P, Londoo ME. In vitro evaluation of confinement, bioactivity, and degradation of a putty type bone substitute. Mater TodayCommun.2021;26:102105. https://doi.org/10.1016/j.mtcomm.2021.102105
- Martini L, Fini M, Giavaresi G, Giardino R. Sheep model in orthopedic research: A literature review. Comp Med. 2001;51(4):292299.
- Olivera L, Antoniac I. Bone substitutes in orthopedic and trauma surgery. In: Bioceramics and Biocomposites: From Research to Clinical Practice. Springer Nature; 2019. p. 341366.
- Owen G, Dard M, Larjava H. Hydoxyapatite/betatricalcium phosphate biphasic ceramics as regenerative material for the repair of complex bone defects. J Biomed Mater Res B Appl Biomater. 2018;106(6):24932512.
- Piccinini M. Porous calcium phosphate granules for biomedical applications. Mater Sci Eng C. 2012;32(2):131147.
- Rosen, C.J., Ackert-Bicknell, C., Rodriguez, J.P. and Pino, A.M., (2009). Marrow fat and the bone microenvironment: developmental, functional, and pathological implications. Critical Reviews in Eukaryotic Gene Expression, 19(2).
- Stiller M, Kluk E, Bohner M, Lopez-Heredia MA, Mller-Mai C, Knabe C. Performance of -tricalcium phosphate granules and putty, bone grafting materials after bilateral sinus floor augmentation in humans. Biomaterials. 2014;35(10):31543163.
- Youssef MM, Bayoumi DA, Hassan HY, Abdelazez YM, Tohamy S. Effect of Milled Tooth Granules versus -tricalcium phosphate as Bone Grafts on Healing of Tooth Extraction Sockets of Rabbits: An Experimental Study. Egypt Dent J. 2022;68(4):3305-3322.
- Zhang LY, Bi Q, Zhao C, Chen JY, Cai MH, Chen XY. Recent Advances in Biomaterials for the Treatment of Bone Defects. Organogenesis.2020;16(4):113-125. https://doi.org/10.1080/15476278.2020.1808428
- Zhou Z, Buchanan F, Mitchell C, Dunne N. Printability of calcium phosphate: calcium sulfate granules for the application of tissue-engineered bone scaffolds using the 3D printing technique. Mater Sci Eng C. 2014;38:1-10.
