Impact of Hydroxyapatite Tooth Paste on Enamel Surface Roughness of Artificially Demineralized Permanent Teeth (An in vitro study).
Abstract
Aims: The current study aimed to evaluate the impact of hydroxyapatite (HA) toothpaste, synthesized from chicken eggshell powder (CESP) on the surface roughness of artificially demineralized enamel, in comparison to sodium fluoride paste via profile projector machine. Materials and Methods: Fifty sound premolars had been split into five groups at random, and artificial caries lesion was made by soaking the samples in the demineralizing solutions, and demineralized surface was cured with: G1 (base line group n=10) without any thing, G2 (control negative group n=10) immersed in demineralizing solution only, G3 (HA paste group n=10) treated with HA paste, G4: (Naf n=10) treated with sodium fluoride paste as control positive, G5: (combination group n=10) treated with HA paste and Naf paste alternatively. The enamel surface roughness was evaluated (at base line, after demineralization, and after treatment. Results: All teeth samples exhibited increased surface roughness after demineralization, in comparison with surface roughness of baseline group, then all treatment groups showed decrease in surface roughness after treatment, except control negative where was deionized water used for storing teeth. Conclusion: HA paste, Naf toothpaste (Flurokin), and a combination of them have remineralizing ability; a decrease in the surface roughness of demineralized enamel surface reflects this.
References
- Abdel-Hakim S M, Metwalli N, El-Askary F, & Wasse M O. Microhardness, SEM and Color change analysis of artificial enamel lesions in primary teeth treated with resin infiltration, cpp-acp or fluoride gel: An in vitro study. Dent. J. 2016; 62: 3744.
- Abdelaziz RH, Mohamed AA and Talaat D M. Effect of two remineralizing agents on microhardness of initial enamel caries like lesions in young permanent teeth.Alexandria Dental Journal. 2019;44(3), 45-49.
- Agus-Imam D N, Dwiandhono I & Mukarromah A. Differences in surface roughness of enamel after whey-extract application and CPP-ACP in post extracoronal-tooth bleaching.Maj. kedokt. gigi Indones. 2018; 4(1):15-21.
- Al-baso A and Al-Nuaimi R. Biomimetic Enamel Remineralization: A Comparative in vitro Study of Self-Assembling Peptide, Fluoride Varnish, and their Combination at Different Times by Surface Microhardness Test.Al-Rafidain Dental Journal. 2022;22(2): 245-254.
- Alhussary BN. Preparation and characterization of natural nano hydroxyapatite from eggshell and seashell and its effect on bone healing. 2020. Ph. D. Thesis. Mosul University, Dentistry College. Mosul. Iraq
- Anil A, Ebrahem W I, Meshni A A, Preethanath R S, Anil S. Nano-hydroxyapatite (nHAP) in the remineralization of early dental caries: A scoping review. International Journal of Environmental Research and Public Health. 2022; 19(9): 5629.
- Attia R M, and Kamel M M. Changes in surface roughness of bleached enamel by using different reminerlizaing agents.Tanta Dental Journal.2016;13(4): 179-186.
- Azih NF, Maqbool M, Halim MS, Noorani TY, Ab Ghani Z. Efficacy, Tooth Surface Microhardness and Roughness after Treatment with Combination of Laser and Home Bleaching Protocol: An in Vitro Cumhuriyet Dent J. 2021; 24(4): 427-441.
- Balan A, Andrian S, Savin C, Sandu A V, Petcu A, Stoleriu S. Comparative study regarding the effect of reminerlizaing products on primary teeth dissolution induced by acidic drinks.Revista de Chime.2015;66: 562-564.
- Featherstone JD and Chaffee BW. The evidence for caries management by risk assessment (CAMBRA). Advance dental research.2018; 29(1): 9-14.
- Gangrade A, Gade V, Patil S, Gade J, Chandhok D, & Thakur D. In vitro evaluation of remineralization efficacy of different calcium-and fluoride-based delivery systems on artificially demineralized enamel surface.Journal of conservative dentistry. 2016; 19(4): 328.
- Hassan N M, Jafar Z J, & Abdul Latif M H. Nanohydroxyapatite preparation for the remineralization of primary tooth enamel surface subjected to liquid medication: An observational study. Health Science Reports.2023; 6(4): e1188.
- Hassan SN and Moharam LM. Effect of Eggshell Powder and Nano-Hydroxyapatite on the Surface Roughness and Microhardness of Bleached Enamel.Contemporary Clinical Dentistry. 2023;14(1): 62.
- Kidd E A. Clinical threshold for carious tissue removal.Dental Clinics. 2010; 54(3): 541-549.
- Natsir N, Tanumihardjm M, Sebon A and Katu H. In situ Effects of Nano hydroxyapatite Paste Derived from Chicken Eggshell on Tooth Enamel during Two Bleaching Regimens. International Journal of Pharmaceutical Research. 2020; (1):699-703.
- Pitek-Jakubek K, Nowak J, Botacz-Rzepkowska E. Influence of infiltration technique and selected demineralization methods on the roughness of demineralized enamel: An in vitro study. Adv Clin Exp Med. 2017; 26(8): 11791188.
- Pitts N B, Zero D T, Marsh P D, Ekstrand K, Weintraub J A, Ramos-Gomez, F, Ismail A. Dental caries.Nature reviews Disease primers. 2017;3(1): 1-16.
- Roveri N, Battistella E, Bianchi C L, Foltran I, Foresti E, Iafisco M, Rimondini L. Surface enamel remineralization: biomimetic apatite nanocrystals and fluoride ions different effects.Journal of Nanomaterials.2009; 1-9.
- Salama F, Abdelmegid F, Al-Sharhan M, Al-Mutairi F, Al-Nasrallah A. Effect of reminerlizaing agents on enamel surface roughness of primary teeth: An in vitro study. EC Dental Science; 2020; 19(2): 01-12.
- Sara AM. The impact of two LASER types in welding the dental enamel fissure system walls: An in-vitro study. M. Sc. Thesis. Mosul University, Dentistry College. Mosul, Iraq 2022.
- Schwendicke F, Frencken J E, Bjrndal L, Maltz M, Manton D J, Ricketts D. ... & Innes N P T. Managing carious lesions: consensus recommendations on carious tissue removal.Advances in dental research. 2016; 28(2): 58-67.
- Shah P, Sharma P, Goje SK, Kanzariya N, Parikh M. Comparative evaluation of enamel surface roughness after debonding using four finishing and polishing systems for residual resin removal :An in vitro study. Prog Orthod. 2019; 20(1):18.
