The Effects of Some Single Bacterial Isolates and Combination between them on Biodegradation of 2- Kirkuk Crude Oil

This study was conducted to show the ability of some isolates of bacteria, Pseudomonas aeruginosa, Bacillus subtilis, Actinomyces spp. and the combination between the three bacterial isolates to biodegrade Kirkuk crude oil. The results indicated that the combination were higher activity on crude oil and reached more than 82%. When Pseudomonas aeruginosa shown biodegradation percent more than 65% on crude oil, But Bacillus subtilis was more than 68% crude oil. The ability of Actinomyces spp. to biodegrade crude oil show more than 45%. When depending by use analysis through Gas-Chromatography and Infra-Red Spectrum furthermore the viable count as another scientific indicators has been added to confirm the occurrence of biodegradation. The results showed that the viable count of the isolates increased with the time of incubation period at 28 days on crude oil, which that confirms the ability of isolates of strains in this study for a highly used of the component of this crude oil in growth.