Comparison of Toxin Fractions Isolated from Protoscoleces and Hydatid Cyst Fluid of Hydatid Cyst of Sheep in Mosul, Iraq

Pooled protoscoleces and hydatid fluids were collected from different sheep livers, and compared with protoscoleces and hydatid fluid from liver of one sheep. Toxin fraction of protoscoleces (P) and hydatid cyst fluid were isolated from hydatid cyst of sheep origin. Chloroform-methanol soluble fractions (CMSFs) were separated by thin layer chromatography (TLC) on silica gel G 60 (Riedel-deHean) coated plate (20X20 cm) on mixture of hexane:diethyl ether:formic acid (80:20:2 v/v/v). CMSFs were identified by TLC separation, ultra-violet light (254 nm) and by iodine evaporation under visible light.
the separation of toxin fractions (TFs) and hydatid cyst fluid (HCF), from different batches of liver protoscoleces and HCF, on TLC, indicate the existence of some differences in the number and rate flow of the TFs between protoscoleces and cyst fluid of the same host and between protoscoleces obtained from cysts of different sheep, the same was obtained in case of HCF of different sheep hosts.