Activity and Properties of 5- Nucleotidase in Human Amniotic Fluid

The present research includes a study of activity and properties of 5 - Nucleotidase (5-NT, EC.3.1.3.5) was determined in human amniotic fluid using coloremetric method. Maximal activity of the enzyme was obtained in a reaction mixture containing 40 mM of Barbiturate-HCl buffer at pH 7.5 containing 11 mM of adenosine 5- monophosphate (5-AMP) as a substrate and 50 l of amniotic fluid (Enzyme volume). The reaction mixture was incubated at 37C for 30min, under the optimum condition. The enzyme activity equal to (66.70.32) unit per liter in amniotic fluid.