Investigate about some of Fatty Acids in Callus Cultures of Corylus avellana L. Induce by Phenylalanine (Phe.)

Oleic, linoleic, palmitic, lauric and stearic acids were isolated and identified in the callus culture of hazelnut Corylus avellana L., which were induced and grown on MS media supplemented with l.5 and l.0 mg/l of BA and 2,4-D respectively, as well as the callus cultures that grown on the same medium in addition to different concentration of Phenylalanine (Phe.), Callus grown on MS medium without addition of growth regulators (MSO) was used as control. Fresh weights of callus were recorded after 40 and 80 days of culture. Gas chromatography was used for identification of the fatty acid, and found that both oleic acid and linoleic acid were the most common in the callus cultures of hazelnut, also found that fatty acids identified in callus culture of hazelnut vary in their types as well as their quantity according to the concentration of Phenylalanine added with plant growth regulator to the medium, and that concentration of fatty acids in callus cultures increased with the increase of callus age