The Relationship of Fatty-Acid Composition of Female Serum to the Level of some Biochemical Variables and the Possibility of Myocardial Infarction

The present study investigates the relationship between fatty-acid composition (in cholesterol ester and phospholipids), the biochemical parameters (total cholesterol, TC; high density lipoprotein-cholesterol, HDL-C; uric acid, malondialdehyde, MDA and glutathione, GSH), the possibility of myocardial infarction and diabetes mellitus in women aged 41-65 years.
Serum samples were divided into two parts. Lipids were extracted from the first part by organic solvents and their compounds were separated by thin-layer chromatyography. Cholesterol ester and phospholipids were hydrolyzed and the produced fatty acids were re-esterified by BF3/methanol. Fatty acid-methyl esters were separated and identified by capillary gas chromatography. It was found that patient groups exhibited a higher level in n6 fatty acids but a lower level in n3 fatty acids and in the ratio of n3/n6 compared with the control group. The second part of serum was used to estimate the biochemical parameters. Uric acid, TC and MDA showed an increase in their levels while HDL-C, GSH and the ratio of HDL-C/TC exhibited a decrease in their levels in the patient groups.
It was concluded that the increase in n6-fatty acids, TC, MDA (the main product of lipid peroxidation) and uric acid( one of the risk signs of having heart artery disease) as well as the decrease in GSH, HDL-C, HDL-C/TC ratio, n3-fatty acids and n3/n6 fatty acids ratio reflect a negative signs of mycocardial infarction and diabetes mellitus.