The Role of Extracted Carotenoid from Staphylococci as Antioxidant and Antibacterial

The ability of some Staphylococci isolates producing and non-producing carotenoid pigments to resist the different concentrations of H 2O 2 and Staphylococcus aureus ranged between 0.6-1.5% studied in dark and light had been studied. The results showed that staphyloxanthin producing strains were more resistant than carotenoid producing pigmented and non-pigmented strains and this resistance was better in dark than light due to the pigment oxidation. Extraction and partial purification of the pigment was done and some biochemical properties such as conductivity and UV-spectra were performed. High conductivity had been registered at 469.25, 391.25 ms besides UV-spectra determination revealed peaks at 450-468 nm for staphyloxanthin and orange- carotenoid pigments respectively. Antibacterial ability of partially purified pigments had been tested against different bacterial isolates, results indicated that partially purified staphyloxanthin had highest antibacterial activity against Staphylococcus epidermidis, Acinetobacter baumanii and Proteus mirabilis, the lowest activity appeared against both Psedumonas aeruginosa and Shigella dysenteri, the other carotenoid pigment on the other hand, had no antibacterial effects against these isolates.