Inhibitory activity of natural phenolic compounds isolated from callus culture of cotyledon leaves of Jatropha curcas L. against pathogenic bacteria

Jatropha curcas L. is characterized by its high content of oil and its ability to grow in different conditions. Also one of the important medicinal plants, as its leaves have been used to treat many diseases. They contain a group of active compounds, including phenols. The current study performed the separation and estimation of phenolic content using HPLC technology from cotyledon callus and follow up its inhibitory activity against pathogenic bacteria. Different types of phenolic compounds were identified in both crude ethanolic and phenolic extract. The highest concentration was reported compound with 4-hydroxy benzoic acid, which reached 239.6957mgL^-1 for the ethanolic crude extract and 286.4765mgL^-1 for the phenolic extract. The lowest concentration was for the Rutin compound that reached 0.0021mgL^-1 for the crude ethanolic extract. The compound Gallic acid, reached 0.1678mgL^-1 for the phenolic extract. The ethanolic and phenolic crude extracts also showed effectiveness against a number of humans and plants pathogenic bacteria. The remarkable effect of the ethanolic crude extract was against Klebsiella pneumoniae bacteria with a diameter of 13 mm of inhibition zone, while the highest inhibitory effect was for the phenolic extract against Agrobacterium rhizogenes 15834 with a diameter of 25 mm of inhibition zone.