Investigation of some Gram-Negative Bacteria from Dental Caries in Diabetic Patients in Al-Muthana

The study aims to determine the extent of the effect of diabetes on the spread of some Gram-negative bacteria that cause tooth decay by comparing their spread with those without diabetes (as a control group). The oral cavity is home to many different types of microbes (all of whom had dental caries under the age group of 1370 years), to find out the extent of the effect of diabetes on increasing the frequency of these bacterial isolates. This study was conducted during the period from August 2023 to January 2024 in Samawah, in the Al-Muthanna Governorate. The specimens of tooth lesions were collected from a total of 100 diabetic patients and 50 non-diabetic patients under sterile conditions. Selective and sub culture media were used to culture and isolate the samples, and four bacterial species belonging to these strains were detected: Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Citrobacter Freunde. Then bacteriological examination was performed. The frequency of bacterial isolates from the diabetic group was higher than that of non-diabetic patients, and their prevalence in women was higher than in males.