Antibiofilm Activity of Mersacidin produced by Bacillus sp.-AE Against Acinetobacter baumannii and Acinetobacter junii isolated from Clinical Specimens.

One hundred and sixty clinical Specimens were collected, of which 120 isolates belonging to genus Acinetobacter spp, for 75%, and the highest percentage of burns was 85.7%, wounds 75.6%, diabetic foot ulcers 75%, tonsil smears 66.6%, urine from cases of urinary tract infection and kidney failure 50%, and bone marrow fluid spiny C.S.F by 25%. from various Mosul hospitals. Thirteen isolates belonging to the two species Acinetobacter baumannii-AE and Acinetobacter junii-AE were selected based on morphological traits and biochemical tests. The phylogenetic relationship based on 16SrRNA The Acinetobacter species clustered in 3 clusters: A: include 8 strains A. baumannii-AE at 98.05%, B: include 2 strains A. junii-AE at 98.2% and C: include 3 strains A. junii-AE at 98.75% were obtained using (Mega7) program and method Clustering depended on (UPGMA). The results showed that the genus Acinetobacter produced a biofilm at 53.8% strongly, while the average production on CRA medium was 46.1%, while the results on MTP 53.8% strong production of the mucous layer in abundance with dark colonies and 46.1% medium producing biofilm with brown colonies, the minimum inhibitory concentration of Mersacidin extracted from strain Bacillus sp.-AE against pathogenic isolates A.baumannii and A.junii ranged between (625-2500) g/ml, while minimum sub MIC ranged between (312.5-1250) g/mL, and the strain Bacillus sp.-AE Mersacidin-producing bacteriocins have the highest anti-biofilm effect than A.baumannii and A.junii strains. is a strong biofilm-forming.