Measurement of Interleukins in Mice Treated with Pro-30 Max Probiotics and Injected with Protoscoleces of Echinococcus granulosus.

Echinococcus granulosus is a causative agent of cystic echinococcosis, with a cosmopolitan allocation. The current study investigated levels of interleukins IL-2, 12, 13 and IL-15 in the serum of mice treated with PRO-30 Max probiotics, comprising (Lactobacillus acidophilus, L. plantarum, L. casei, L.reuteri, Bifidobacterium bifidum, B. lactis, B. longum, and Streptococcus thermophilus), then infected with the protoscoleces of E. granulosus. Three dilutions of probiotics, 1810-8/1 ml, 1410-9 / 1 ml, 12 x 10-10/ ml (CFU), and three periods 3, 5, and 7 months were applied, 0.1 ml of each dilution was injected in mice intraperitoneally, before and after infection with the protoscoleces. On the fifth day of infection, bacterial injection was continued every 72 hours for all periods. Blood was drawn from the ophthalmic venous plexus; serum samples were kept at -20 until use. Interleukins were estimated using ELISA technique. The highest level of IL-2 was at dilution 1810-8, 195.29 pg/ml, compared with the C+, 30.212 pg/ml after seven months of infection. The highest level of IL-12 was at dilution 1810-8, 195.19 pg/ml, compared with the C+, 35.955 pg/ml after seven months of infection. The lowest level of IL-13 in mice treated with the dilution 1810-8, was 31.755 pg/ml, after three months of infection, compared with the C+, 190.919 pg/ml after the seventh month of infection. An increase in the level of IL-15 was observed at the dilution 18 10-8, 193.012 pg / ml, compared with the C+, 30.022 pg/ ml after seven months of infection.