The Purification of Maize Dwarf Mosaic Virus (MDMV) and Preparation of its Antiserum

Maize dwarf mosaic virus (MDMV) was diagnosed on corn crop in Ninewah province by using tissue blot immunoassay (TBIA). The virus isolate was propagated in corn plants. The virus was purified by differential centrifugation and precipitated by polyethylene glycol (PEG) and then by sucrose density gradient. The efficiency of purification method was evaluated biologically and spectrometrically, and virus purity was 1.12 . Its antiserum was prepared by using rabbit, the end the dilution point ( titer ) of antiserum was 1/ 512. The efficiency of antiserum was tested by agglutination, and agar double diffusion tests .