The Effect of Aqueous Extracts of Nerium oleander and Melia azedarach on Growth of Trichomonas vaginalis In vitro

The present study involved the poisonous effect of an aqueous extract of Nerium oleander leaves and Melia azedarach fruits on Trichomonas vaginalis, growing on Diamonds medium (TYM) enriched with 10% of inactivated human plasma.
The results indicated that these extracts have poisonous effect on T. vaginalis. The uses of concentrations 0.5, 1, 1.5, 1.75, 2, 2.5 mg/ml for leaves extract of N. oleander and 0.5, 1, 1.5, 1.75, 2 mg/ml for fruits extract of M. azedarach, caused gradual inhibition of trichomonad numbers proportioned with the increase of concentration during different growth periods 24-48-72 hours.
The high concentrations 2.5 mg/ml for leaves extract of N. oleander and 2 mg/ml for fruits extract of M. azedarach caused inhibition of the trichomonads growth at rates 66.6% and 68.8% respectively during 72 hours of growth.
It was observed that these extracts have an effect on number of generation which decreased during different growth periods between 0.260.12 to 1.570.22 and 0.4 0.08 to 1.530.13 generation in contrast with control 1.840.14 to 3.170.04 for N. oleander and 1.480.13 to 3.220.06 for M.azedarach_when high concentrations of N. oleander leaves and M. azedarach fruits extracts were used respectively. It was also observed that these extracts had an effect on generation time which increased with increasing of concentrations used during different growth periods.
The IC50 concentrations for extracts were determined which were 2 and 1.75 mg/ml for N. oleander and M. azedarach, respectively, during 72 hours of growth.