Isolation and Diagnosis of Some Auxins and Cytokinines in Seedlings and Callus of Nigella sativa L.

This study included detection the content of callus and seedlings of
Nigella sativa L. IAA (Indole-3-acetic Acid) and 2,4-D (2,4-Dichlorophenoxyacetic Acid) of Auxins, BA (Benzyl Adenine) and kinetin of the cytokinines, the pure extraction of callus and seedlings were prepared by the use of 80 % Ethanol, diagnosis of plant growth regulators was carried out by using thin layer chromatography technique (TLC) which proved that Rf values of spots separated from callus were (0.97 / 0.62 / 0.96) which were nearly to that Rf values of the spots of standards of IAA, 2,4-D and BA which were (0.95 / 0.67 / 0.96) respectively. Also the results showed that the Rf values of spots separated from seedling extract were (0.94 / 0.78 / 0.95) which were nearly that Rf of IAA, BA and kinetin (0.95 / 0.74 / 0.96) respectively.
colorimetric method depended on salkoweski reagent was used for estimation of IAA in callus and seedling extracts of Nigella sativa, the results showed that seedling content of IAA almost reached to 20 Mg/g fresh weight, while callus extract gives 11 Mg/g fresh weight.