Studying Some of the Extracts and Isolated Proteinous Compounds From Apple (Pyrus malus) in Mice Exposed to Oxidative Stress

This study was conducted to prepare cold and boiled aqueous extracts of Pyrus malus fruits, and thens to isolate extracts of alkaloid, flavonoids and glycosides for this plant. The study also comprised the sisolation and study the proteinous compounds, one of the techniques used to isolation was the gel filtration technique which was isolated two compounds A (166226) Dalton, B (2925) Dalton from the cold proteinous precipitate and two compounds A (34609) Dalton, B (719) Dalton from the boiled proteinous precipitate. The effects of these extracts and compounds previously mentioned above were studied on serum glucose, total cholesterol, total lipids, low density lipoprotein-cholesterol (LDL-C) and high density lipoprotein-cholesterol (HDL-C) levels, also glutathione (GSH) and malondialdehyde (MDA) levels in liver, kidney and heart tissues in mice exposed to oxidative stress. These extracts were administrated intraperitoneally.
After one week from the treatment the results were indicated that the cold and boiled crude aqueous, non proteinous extracts, proteinous precipitate and proteinouse compounds (B) which were isolated from it at the doses of 500,500,490.35, 491.66, 9.65,8.34,5.07,3.836) mg/kg body weights ,also the alkaloids, flavonoids at the doses of (0.52,0.31) mg/kg body weights were caused a significant decrease (p<0.05) in serum glucose, total cholesterol, total lipids, LDL-C levels and MDA level in liver, kidney and heart tissues, with an associated significant increase (p<0.05) in serum HDL-C level and GSH level in liver, kidney and heart tissues in mice exposed to oxidative stress. Finally we suggested most of extracted isolated from fruits of apple (especially the extract of flavonoids) have antioxidant in mice exposed to oxidative stress.