Culture of Different Densities of Cell Suspensions Derived from Stem Callus of Broadbean (Vicia faba) in Agar Sector and Regeneration of Intact Plants

The study succeeded in establish cell suspension derived from stem callus of the local variety of axenic broad bean (Vicia faba L.) seedlings. Different densisties (5.6, 4.8, 4.2, 3.7, 3.4, 3.3, 3.2, 3.0, 2.9 103 cell/ml) of these suspensions were cultured by embedding them in sectors of agar-solidified MS medium in the presence of different levels of naphthaleneacetic acid (NAA) and benzyl adenine (BA). This method of culture promote division of embedded cells and enhance colony formation to reach up to 67%. They developed to form callus primordia, varied in their formation ratio between 30-50% depending upon culture density.
The produced calli showed a groth pattern superior the growth pattern of calli stimulated from stem explant. Moreover, the callus express its capability to regenerate plants. Number of shoots ranged between 5-7 shoots per piece of callus.