Labrotary Prepared Trizoles Derivatives Used Instead of Standard Cytokinin in the Initiation and Growth of Single Cells and Suspension Culture of Lettuce Plant (Lactuca sativa L.)

It was found that different derivatives from Triazoles produced locally can be used instead of the imported cytokinins in the medium for initiation of cell suspension and single cell of lettuce plant.
Addition of NAA to MS media containing the triazoles derivative enhanced greatly the initiation of cell suspension and division better than the effect of standard growth regulators. Moreover, these compounds sustain the cell division in the systems used. The density of the cells in the suspension medium reached 21.1 X 103 cell/cm3 in the presence of compound E, 17.0 X 10-3 cell/cm-3 with compound G and 11.1 X 103 cell/cm3 with compound F in contrast with 8.8 X 103 cell/cm3 in the standard medium after 6 days of growth.
The results indicate that addition of these compounds increased greatly the viability of the single cell system in the same way as in the case of the standard medium. Addition of these compounds instead of standard cytokinins with NAA stimulates the growth of the cells grown on Agar-Drop system with the formation of callus with the lapse of growth period. The initiation of callus by this method was between18 to 47 % as compared with 63% in the standard medium.
It can be concluded that these prepared compounds can be used as a new growth regulators instead of the standard cytokinins. These compounds can be used in the other tissue culture system for different plants