Determination of the Changes in Human Leukocytes Inflammatory Genes Expression after Infection with Human Papillomavirus

Keratinocytes are the natural target cells for infection by humanpapillomaviruses (HPVs), most of which cause benign epithelial hyperplasias (warts). HPVs, cause lesions that can progress to carcinomas. Inflammatory genes such as those coding for cytokines, chemokines and other genes are induced in response to a viral infection. HPV effect on host cellular responses is critically important to explore the molecular mechanisms of viral-host interaction. The aims of this study were to detect changes in the mRNA expression in a panel of 84 inflammatory genes in the leukocyte of three HPV infected patients using real time-PCR array technology. Nine inflammatory genes showed a significant gene up regulation ranging between 16.897 mean fold change for Interleukin-1 alpha and 353.804mean fold change for Interferon-alpha2 gene. The up regulation of mRNA of nine geneswith high significance (P > 0.05) might suggest that the host-viral interaction is a cellular response against viral effect. In conclusion,this study revealed several alterations of many Leukocyte`s inflammatory gene expressions as a response against HPV. The common up regulated genes after HPV infections stimulate the activity of different immunological functional pathways represented by cytokine-cytokine receptor interaction, NOD-like receptor signaling pathway, and Toll-like receptor signaling pathway.