Partial Purification of 5´-Nucleotidase Isoenzymes from Blood Sera of Anemic Patients

In this study, 5-Nucleotidase isoenzymes were partially purified from blood sera of anemic patients, through different steps including: Protein precipitation with ammonium sulfate and dialysis. Three isoenzymes were isolated from dialyzed protein fraction by using Anion-exchange chromactography (DEAE-Cellulose A-50), with maximum purification folds (22.13) for isoenzyme . In addition, the kinetic studies for the isolated isoenzymes were carried out, which included the effect of different substrate (5- AMP) concentration and determination their Km values: 14.08, 12.98 and 8.4 (mM) respectively compared to normal; 13.0, 14.08 and 15.6 (mM).