Isolation and Identification of Brevibacterium Species from Dairy Product and some Clinical Specimens
Abstract
Isolation and identification of the member of the genus Brevibacterium which belong to Actinomycetes, were carried out, a total of (175) sample from different sources of dairy products, human blood and skin from kidney dialysis and cancer patient from August 2010 to February 2011.
The result indicate the higher percentage of isolation from dairy (32%), cheese and milk (20%) for each of them, blood of kidney dialysis 12% (8% male and 4% female)and from skin of kidney dialysis patient (4%), and they didnt isolated from skin and blood of cancer patient.
The isolates were purified on the modified media Plate Count Agar (PCA) and blood agar and was purified and then identified to species level depending on the morphological, characteristics as shape, color and consistency of colony, sensitivity to antibiotics, most of species have shown sensitivity to Ciprofloxacin 5Mg/disc and resistance to penicillin and ampicillin 10Mg/disc, in addition to biochemical and physiological tests to the following
species B. linens, B. epidermidis, B. otitidis, B. iodinum, B. casei, B. sanguinis and
B. paucivorans.