Isolation and Identification of Bacteria Contaminating the Operating Theatres

The study aimed to isolate and identify the types of bacteria
contaminating the environment of operating theaters of three hospitals in Mosul (AL-Khansa, AL-Zahrawi and Saddam General). (802) different environmental hospital specimens were taken, (531) samples were positive and 600 bacterial isolates were isolated and diagnosed using morphological and biochemical tests into (310) isolates (51.68%) of Gram negative bacilli, which included:
1. Klebsiella (15%)
2. Enterobacter (11.33%)
3. Pseudomonas (6%)
4. Escherichia (4.66%)
5. Serratia (4%)
6. Alcaligenes (3.33%)
7. Proteus (2.33%)
8. Citrobacter (2%)
9. Hafnia (1.33%)
10. Providenciae (1%)
11. Morganella (0.66%)
(190) isolates (31.66 %) of Gram positive Cocci which included Staphylococcus (29.16%) which were divided into seven species with Staph. aureus being the dominant (9.5%), while the Streptococci comprised (2.5%) which included Streptococcus faecalis (1.5%) and Streptococcus agalactiae and (100) isolates (16.66%) of Gram positive bacilli which included three species B. subtilis (8.16%), B. cereus (5.33%) and B. coagulans (3.16%).