Direct Determination of Tetracycline Hydrochloride and the Interaction with Bovine Serum Albumin by UV Spectra in Water

The UV fourth order derivative spectra were selected as the more reliable technique for the direct quantification of pure Tetracycline Hydrochloride in water, after the measurement of the zero, first, second, third and fourth order spectra, within the concentration range at (0.2885-144.27)g/ml with R2 =0.9998 and RSD=0.215 %.
Bovine serum albumin was directly quantified by the fourth order UV derivative technique within the concentration range (6.7-2680) g/ml with R2 = 0.9998 and RSD=0.324 %. Appreciable interaction between Tetracycline hydrochloride and Bovine serum Albumin was found in accordance with the fraction coefficient and the apparent binding constant.
The UVfourth order derivative technique appears to be in a good accuracy and precise method for the direct quantification of Tetracycline Hydrochloride and Bovine Serum Albumin (BSA) in water.