Detection the Effect of Cu++ on Transcription of glox Gene in Myceliophthora verrucosa Using RT-PCR
Abstract
Reverse transcriptase PCR was used to detect the transcription of the glox gene in a local isolate of M. verrucosa, in two different growth media. The results indicated the presence of gene transcription in this strain with no effect to Cu+ on gene expression. This means that this fungus is suitable for use in various fields such as a bioremediation. This method depends on RNA to detect gene expression under various conditions.