Polarographic Measurement of Lactate Dehydrogenase Activity in Human Serum “Clinical Application

The paper reports a sensitive and rapid method for the measurement of Lactate Dehydrogenase activity (EC 1.1.1.27) in human serum. The method is based on the polarographic measurement in the reduction of polarographic peak of NAD+ via the enzymatic reaction of LDH with lactate and NAD+.
The method was successfully applied to measure LDH activity in serum of normal and diseased individuals after the removal of albumin by zinc acetate method.